MOLECULAR IDENTIFICATION OF ENDOPHYTIC FUNGI ISOLATED FROM HYSSOPUS OFFICINALIS
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Abstract
In recent years, endophytic fungi of medicinal plants have gained considerable attention as potential sources of novel bioactive compounds. These microorganisms, which reside asymptomatically within plant tissues, are known to produce a wide range of secondary metabolites with antimicrobial, antifungal, and antioxidant properties. In this study, a highly active endophytic fungal isolate was obtained from the vegetative organs of Hyssopus officinalis and subjected to molecular identification. Using the ITS (Internal Transcribed Spacer) region sequencing and BLAST analysis against the NCBI GenBank database, the isolate was identified as Colletotrichum elatum. DNA extraction and sequencing were conducted using CTAB method, PCR amplification with ITS5-ITS4 primers, and Sanger sequencing. The obtained sequence was submitted to GenBank under the accession number OP476344.1. A phylogenetic tree was constructed using MEGA 11 software to confirm taxonomic affiliation. This study reports for the first time the isolation of C. elatum from H. officinalis, underlining its potential as a novel source of bioactive compounds.
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