DETERMINATION OF GLUCOSE CONTENT IN JUICES OF DRIED FRUITS AND VEGETABLES (RAISINS, DRIED APRICOTS, BEETROOT) USING THE AUTOMATIC REFRACTOMETER RX-5000
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Abstract
In this article, a methodology for determining the glucose content in juices of dried fruits and vegetables – namely raisins, dried apricots and beetroot – using the automatic refractometer RX-5000 was developed and experimentally tested. First, the instrument was calibrated using distilled water and standard glucose solutions, the functional relationship between the refractive index (nᴅ) and glucose concentration was established, and this relationship was stored in the memory of the RX-5000 as a user scale. Aqueous solutions were prepared from dried fruit (raisins, dried apricots) and beetroot juice samples in specified ratios, and refractometric measurements were carried out under stable conditions at 20 ± 0.1 °C. The obtained nᴅ values were converted into glucose concentration using the calibration graph, and the results were compared with those of a classical chemical method (titrimetry). According to the comparison, the relative error of the refractometric method based on the RX-5000 did not exceed 2–3 %, and the correlation coefficient was in the range of 0.98–0.99, which indicates the high accuracy and reproducibility of the methodology. The proposed method is recommended as a rapid, low-labor and convenient analytical technique for determining glucose in dried fruit and vegetable juices, suitable for use in educational laboratories.
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