MODERN GENE EDITING METHODS
DOI:
https://doi.org/10.55640/Keywords:
gene editing, CRISPR, Cas9, TALEN, genome engineering, biotechnology, gene therapyAbstract
This article discusses the theoretical foundations, practical applications, and significance of modern gene editing technologies (CRISPR/Cas9, TALEN, ZFN, and others) in the fields of biology and medicine. The advantages and limitations of these methods are analyzed in relation to the treatment of genetic diseases, the improvement of crop varieties, and the optimization of animal breeding.
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References
1.Doudna, J.A., & Charpentier, E. (2014). The new frontier of genome engineering with CRISPR–Cas9. Science, 346(6213), 1258096.
2.Barrangou, R., & Doudna, J. (2016). Applications of CRISPR technologies in research and beyond. Nature Biotechnology, 34(9), 933–941.
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4.Joung, J.K., & Sander, J.D. (2013). TALENs: a widely applicable technology for targeted genome editing. Nature Reviews Molecular Cell Biology, 14(1), 49–55.
5.Zetsche, B. et al. (2015). Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell, 163(3), 759–771.
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Authors retain the copyright of their manuscripts, and all Open Access articles are disseminated under the terms of the Creative Commons Attribution License 4.0 (CC-BY), which licenses unrestricted use, distribution, and reproduction in any medium, provided that the original work is appropriately cited. The use of general descriptive names, trade names, trademarks, and so forth in this publication, even if not specifically identified, does not imply that these names are not protected by the relevant laws and regulations.
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