EFFECT OF A COMBINED PREPARATION ON THE ANTIOXIDANT SYSTEM OF RAT LIVER MITOCHONDRIA IN STREPTOZOTOCIN-INDUCED DIABETES

Abstract

Diabetes mellitus is a chronic metabolic disorder characterized by persistent hyperglycemia and increased oxidative stress, which plays a key role in the development of diabetic complications. Streptozotocin (STZ)-induced diabetes is widely used as an experimental model to investigate metabolic and mitochondrial dysfunctions associated with diabetes. In this study, the effect of a combined preparation on the antioxidant defense system of rat liver mitochondria was evaluated under STZ-induced diabetic conditions. The activity of key antioxidant enzymes, including superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx), was assessed using spectrophotometric methods.

The results demonstrated a significant decrease in mitochondrial antioxidant enzyme activities in diabetic rats, indicating pronounced oxidative stress and mitochondrial dysfunction. Treatment with the combined preparation significantly improved SOD, catalase, and GPx activities, suggesting restoration of the antioxidant defense system and reduction of oxidative damage. In healthy animals, the combined preparation did not induce significant biochemical changes, indicating its safety under physiological conditions.

These findings suggest that the combined preparation may have a protective effect on mitochondrial function by enhancing antioxidant defense mechanisms in diabetic conditions.