HPLC QUANTITATIVE ANALYSIS OF SIPROFLOKS-АК 250 TABLETS (CIPROFLOXACIN): METHOD DEVELOPMENT, VALIDATION AND ASSAY

Authors

  • Turg’unov Dilshod MSc Pharmaceutical Sciences, lecturer Department of Pharmaceutical Sciences of Kokand University Andijan Branch/Professor of the Department of Industrial Technology of Medicines of the Tashkent Pharmaceutical Institute

DOI:

https://doi.org/10.55640/

Keywords:

Ciprofloxacin; Siprofloks-АК; RP-HPLC; method validation; ICH Q2(R1); tablet assay; isocratic

Abstract

A reversed-phase HPLC (RP-HPLC) method was developed and validated for the quantitative determination of ciprofloxacin in Siprofloks-АК tablets. Separation was achieved on a C18 column using an isocratic mobile phase composed of phosphate buffer (pH 3.0, 0.025 M) and acetonitrile; detection was performed at 278 nm. The method was validated according to ICH Q2(R1) for specificity, linearity, accuracy, precision, limit of detection (LOD) and limit of quantitation (LOQ). Linearity was observed over 5–50 µg·mL⁻¹ (r² ≥ 0.999), recovery ranged 98.5–101.3% and intra-/inter-day precision RSD was ≤1.8%. The method is simple, precise and suitable for routine quality control of ciprofloxacin tablets such as Siprofloks-АК. (ICH Database)

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References

1.ICH Q2(R1) — Validation of Analytical Procedures: Text and Methodology. International Conference on Harmonisation. (Accessed via ICH database). (ICH Database)

2.Scherer R, et al. Determination of Ciprofloxacin in Pharmaceutical Formulations by RP-HPLC. J Chromatogr A / related publication (example method using XBridge C18, detection at 278 nm). PMC article. (PMC)

3.Ali SA, et al. An RP-HPLC method for the determination of Ciprofloxacin HCl. (method development and validation; detection at 278 nm, buffer/ACN mobile phases). Journal / report (2011). (JAPS)

4.Vaghela BK, et al. A novel validated stability-indicating HPLC method for quantitative determination of ciprofloxacin and tinidazole impurities. Journal / PMC (2013). (PMC)

5.Shohag MH, et al. Post-market quality assessment of 22 ciprofloxacin brands using validated RP-HPLC. ScienceDirect (2023). (sciencedirect.com)

6.Goyal A., Anju et al. A Validated RP-HPLC Method for Estimation of Ciprofloxacin and Tinidazole in Combined Tablet Dosage Form. International Journal of Pharmaceutical Chemistry and Analysis (2015). (SciSpace)

7.Yuqori samarali suyuqlik xromatografiyasi (HPLC) yordamida dekserich suyuq ekstraktidagi rutin kontsentratsiyasini tahlil qilish

8.M Axmatoxunova, A Shokirov - Journal of Integrated Education and Research, 2024

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Published

2025-11-17

Issue

Vol. 4 No. 10 (2025): Journal of Multidisciplinary Sciences and Innovations

Section

Articles

License

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Authors retain the copyright of their manuscripts, and all Open Access articles are disseminated under the terms of the Creative Commons Attribution License 4.0 (CC-BY), which licenses unrestricted use, distribution, and reproduction in any medium, provided that the original work is appropriately cited. The use of general descriptive names, trade names, trademarks, and so forth in this publication, even if not specifically identified, does not imply that these names are not protected by the relevant laws and regulations.

How to Cite

HPLC QUANTITATIVE ANALYSIS OF SIPROFLOKS-АК 250 TABLETS (CIPROFLOXACIN): METHOD DEVELOPMENT, VALIDATION AND ASSAY. (2025). Journal of Multidisciplinary Sciences and Innovations, 4(10), 1178-1181. https://doi.org/10.55640/

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