STUDY OF THE WOUND-HEALING ACTIVITY OF A HYDROGEL IMMOBILIZED WITH 6-AMINOPURINE
DOI:
https://doi.org/10.5281/zenodo.20283088Keywords:
6-Aminopurine; hydrogel; wound healing; tissue regeneration; veterinary medicine; controlled release; epithelialization; livestock wounds.Abstract
Rapid healing of traumatic and postoperative wounds in farm animals remains an important veterinary problem due to infection risk, dehydration of tissues and slow regeneration under field conditions. In this study a bioactive hydrogel containing immobilized 6-aminopurine was developed and its wound-healing activity was evaluated. The hydrogel matrix was obtained from biocompatible polysaccharides followed by ionic crosslinking and incorporation of the active compound to ensure prolonged release. Physicochemical properties of the material (swelling capacity, moisture retention and biodegradability) were characterized, and release kinetics of 6-aminopurine from the matrix were determined in physiological medium.
Biological activity was assessed on experimental skin wounds in farm animals by monitoring inflammation phase duration, granulation tissue formation, epithelialization rate and microbial contamination. Application of the hydrogel created a moist protective environment, reduced exudation and prevented secondary infection. Compared with untreated control wounds, the treated group demonstrated faster granulation, earlier epithelial layer formation and shorter healing period. The regenerating tissues showed reduced edema and more organized collagen fibers.
The results indicate that immobilization of 6-aminopurine in a hydrogel matrix provides controlled delivery of the bioactive compound and significantly enhances reparative processes. The developed preparation can be considered a promising antibiotic-free regenerative veterinary material for treatment of wounds and inflammatory skin lesions in livestock.
Downloads
References
1.Orinbayevna, B. G., & Ergashevich, S. F. (2026). Study of the selective acylation and physicochemical properties of 6-benzylaminopurine. Universum: химия и биология, 4(1 (139)), 46-50.
2.GO, B., & Saitkulov, F. E. Study of the selective acylation and physicochemical properties of 6-benzylaminopurine.
3.Dilshod odil o’g’li, K., Ergashevich, S. F., & Shamshetovich, T. M. (2025, November). Improving the criteria for detection and certification of pesticide residues in fruits and vegetables in uzbekistan. In Conferences (Vol. 1, No. 4, pp. 640-643).
4.Fotima, Q., Foziljon, S., Jalgasbayevna, G. U., & Shamshetovich, T. M. (2025, November). Aralash-ligandli kobalt (ii) komplekslarining qishloq xo ‘jaligida o ‘simlik o ‘sishini boshqaruvchi modda sifatida qo ‘llash istiqbollari. In Conferences (Vol. 1, No. 4, pp. 583-586).
5.Ilmpazovna, H. D. (2025, October). Technology for enhancing the uptake of iron and zinc elements in medicinal plants using hydrogel capsules. In London International Monthly Conference on Multidisciplinary Research and Innovation (LIMCMRI) (Vol. 2, No. 1, pp. 826-829).
6.Usmanovich, E. T., Oxunov, I. I., & Shamshetovich, T. M. (2025, November). 6-Aminopurinning qahrobo kislotasi bilan ta’sir reaksiyasini o ‘rganish, sintez qilish texnologiyasi. In Conferences (Vol. 1, No. 4, pp. 649-653).
7.Maxmarajabovich, X. M., Ergashevch, S. F., & Suvanovich, X. T. Y. (2024). The use of information technologies in teaching biophysics and radiobiology. Science and innovation, 3(Special Issue 58), 522-526.
8.Shoyimovich, K. G., Ergashevich, S. F., & Kuchkar, G. (2024). Determination of certain heavy metals in food composition by voltammetric method. Austrian Journal of Technical & Natural Sciences.
9.Saitkulov, F., Begimqulov, I., Oʻralova, N., Gulimmatova, R., & Rahmonqulova, D. (2022). Biochemical effects of the coordination compound of cobalt-II nitrate quinazolin-4-one with 3-indolyl acetic acid in the “amber” plants grades phaseolus aureus. Академические исследования в современной науке, 1(17), 263-267.
10.Saitkulov, F., Farhodov, O., Olisheva, M., Saparboyeva, S., & Azimova, U. (2022). Chemical feeding method of lemon plant using leaf stomata. Академические исследования в современной науке, 1(17), 274-277.
11.Saitkulov, F., Sapaev, B., Nasimov, K., Kurbanova, D., & Tursunova, N. (2023). Structure, aromatic properties and preparation of the quinazolin-4-one molecule. In E3S Web of Conferences (Vol. 389, p. 03075). EDP Sciences.
12.Saitkulov, F., Zakhidov, Q., Khaydarov, G., Sabirova, D., Ergasheva, H., Mirvaliev, Z., & Usnatdinova, S. (2025, February). Methods for the synthesis of 2-phenylquinazolin-4-one and studying methylation reactions in different solvents. In AIP Conference Proceedings (Vol. 3268, No. 1, p. 030038). AIP Publishing LLC.
13.Kulmirzayeva, S., Isaqulova, M., Nasimov, H., Saitkulov, F., & Islomova, D. (2025, July). Study of synthesis and biological properties of coordination compound of cobalt (II)-chloride. In American Institute of Physics Conference Series (Vol. 3304, No. 1, p. 040099).
14.Saitkulov, F., Abdullayev, F., Xudayrov, M., Eshboboev, T., & Haydarov, G. (2024). Technology for detecting heavy metals in the soil using an ionometer. In BIO Web of Conferences (Vol. 105, p. 05004). EDP Sciences.
15.Gulbaxar, B. (2025). OPTIMAL SYNTHESIS OF QUINAZOLIN-4-ONE. Universum: химия и биология, 2(2 (128)), 31-33.
16.Saitkulov, F. E. (2024). STUDYING THE REACTION OF BAP WITH SUCCINIC ACID AND ITS EFFECT ON THE ROOTING OF THE SEEDLING OF THE VARIETY “BUKHARA-102”. Austrian Journal of Technical and Natural Sciences, (1-2), 13-18.
17.Bekboyevich, O. O., Ergashevich, S. F., & Zoxidovich, M. Z. (2024). SYNTHESIS REACTIONS OF QUINAZOLIN-4-ONE IN THE PRESENCE OF IRON (III)-CHLORIDE CATALYSTS. Austrian Journal of Technical and Natural Sciences, (9-10), 49-53.
18.Bekboyevich, O. O., Ergashevich, S. F., Zoxidovich, M. Z., & Orinaevna, B. G. (2024). INVESTIGATION OF AROMATIC PROPERTIES OF XINAZOLIN4-ONE. Austrian Journal of Technical and Natural Sciences, (9-10), 54-57.
19.Saitkulov, F. E., & Elmuradov, B. (2024). Zh., Sapaev B. Syntheses and biological activity of quyinazolin-4-one hydrochloride. Austrian Journal of Technical and Natural Sciences, (1-2), 28-35.
20.Sapayev, B., Saitkulov, F. E., Normurodov, O. U., Haydarov, G., & Ergashyev, B. (2023). Studying Complex Compounds of Cobalt (II)-Chlooride Gecsacrystolohydrate with Acetamide and Making Refractory Fabrics from Them.
21.Saitkulov, F. E., Giyasov, K., & Elmuradov, B. J. (2022). Methylation of 2-methylchiazoline-4-one by" soft" and" hard" methylating agents. Universum: Chemistry and Biology,(11–2 (101)),–49 c.
22.Saitkulov, F. E., Giyasov, K., & Elmuradov, B. J. (2022). Methylation of 2-methylchiazoline-4-one by" soft" and" hard" methylating agents. Universum: Chemistry and Biology,(11–2 (101)),–49 c.
23.Saitkulov, F., Azimov, I., Ergasheva, M., & Jo‘raqulov, H. (2022). Carbohydrates are the main source of energy in the body. Solution of social problems in management and economy, 1(7), 68-71.
24.Khatamov, K., Saitqulov, F., Ashurov, J., & Shakhidoyatov, K. (2012). 3, 5, 6-Trimethylthieno [2, 3-d] pyrimidin-4 (3H)-one. Structure Reports, 68(9), o2740-o2740.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors retain the copyright of their manuscripts, and all Open Access articles are disseminated under the terms of the Creative Commons Attribution License 4.0 (CC-BY), which licenses unrestricted use, distribution, and reproduction in any medium, provided that the original work is appropriately cited. The use of general descriptive names, trade names, trademarks, and so forth in this publication, even if not specifically identified, does not imply that these names are not protected by the relevant laws and regulations.
Germany
United States of America
Italy
United Kingdom
France
Canada
Uzbekistan
Japan
Republic of Korea
Australia
Spain
Switzerland
Sweden
Netherlands
China
India
